Journal:

Article Title: Analysis of Virulence and Inflammatory Potential of Shigella flexneri Purine Biosynthesis Mutants

doi: 10.1128/IAI.71.12.7002-7013.2003

Figure Lengend Snippet: Primers used in RT-PCR analysis

Article Snippet: The primary monoclonal antibodies (MAbs) employed were the following: a rat anti-mouse MHC-II MAb (Serotec, Oxford, United Kingdom), a rat anti-mouse tumor necrosis factor alpha (TNF-α) MAb (Serotec), and a murine anti- S. flexneri 5a lipopolysaccharide (LPS) MAb (6 mg/ml) ( 30 ).

Techniques: Sequencing

Journal:

Article Title: Analysis of Virulence and Inflammatory Potential of Shigella flexneri Purine Biosynthesis Mutants

doi: 10.1128/IAI.71.12.7002-7013.2003

Figure Lengend Snippet: RT-PCR and densitometry of products of RNAs extracted from lungs of three mice infected with either M90T or mutants, using primers specific for β-actin, IL-1β, IL-6, IL-12, IFN-γ, TNF-α, and iNOS at 72 h p.i. (A) Control, uninfected control lung; purHD, ZB2209 (M90T ΔpurHD); guaBA purEK, ZB504 (M90T ΔguaBA ΔpurEK); purHD purEK, ZB503 (M90T ΔpurHD ΔpurEK). (B) Control, uninfected control lung; guaBA, ZB501 (M90T ΔguaBA); guaBA purHD, ZB502 (M90T ΔguaBA ΔpurHD). Similar results were obtained in three identical experiments. Standard deviations for three experiments were within 10% of the values of the arbitrary units (a.u.).

Article Snippet: The primary monoclonal antibodies (MAbs) employed were the following: a rat anti-mouse MHC-II MAb (Serotec, Oxford, United Kingdom), a rat anti-mouse tumor necrosis factor alpha (TNF-α) MAb (Serotec), and a murine anti- S. flexneri 5a lipopolysaccharide (LPS) MAb (6 mg/ml) ( 30 ).

Techniques: Reverse Transcription Polymerase Chain Reaction, Infection

Journal:

Article Title: Analysis of Virulence and Inflammatory Potential of Shigella flexneri Purine Biosynthesis Mutants

doi: 10.1128/IAI.71.12.7002-7013.2003

Figure Lengend Snippet: Avidin-biotin immunoperoxidase labeling of TNF-α (A, B, C, and D) and of MHC-II complexes (E, F, G, and H) in tissue sections of lungs of mice infected with M90T (A and E), ZB503 (M90T ΔpurHD ΔpurEK) (B and F), ZB501 (M90T ΔguaBA) (C and G), and ZB502 (M90T ΔguaBA ΔpurHD) (D and H) at 72 h p.i. In panel A arrows point to high expression of TNF-α in neutrophil aggregates, in panels E and F arrows indicate a few MHC-II-expressing cells, in panel G arrows point to some MHC-II-expressing bronchiolar cells and arrowheads point to BALT MHC-II-positive cells, and in panel H some bronchiolar mucosal and mononuclear MHC-II-expressing cells are indicated by arrows and arrowheads, respectively. Bars, 25 μm (A, B, C, and D) and 50 μm (E, F, G, and H).

Article Snippet: The primary monoclonal antibodies (MAbs) employed were the following: a rat anti-mouse MHC-II MAb (Serotec, Oxford, United Kingdom), a rat anti-mouse tumor necrosis factor alpha (TNF-α) MAb (Serotec), and a murine anti- S. flexneri 5a lipopolysaccharide (LPS) MAb (6 mg/ml) ( 30 ).

Techniques: Avidin-Biotin Assay, Labeling, Infection, Expressing

Journal: Journal of the American Society of Nephrology : JASN

Article Title: Cellular and Molecular Mechanisms of Kidney Injury in 2,8-Dihydroxyadenine Nephropathy

doi: 10.1681/ASN.2019080827

Figure Lengend Snippet: Primary antibodies for immunohistochemistry or immunofluorescence staining

Article Snippet: Additional histology samples were stained with van Kossa or silver stain, or immunohistochemistry against keratin, CD68, Ki-67, or TNFR1. table ft1 table-wrap mode="anchored" t5 Table 2. caption a7 Target Protein Antibody Company α -SMA Mouse-anti-human SMA (clone 1A4) Dako/Agilent, M085101–2 (US) Aquaporin-2 Rabbit-anti-AQ2 (polyclonal) Abcam, ab15116 (UK) CD3 Rat-anti-human CD3 (cloneCD3–12) Bio-Rad, MCA1477 (GER) CD11b Rat-anti-mouse CD11b (clone M1/70) PE-labeled eBioscience/Thermo Fisher Scientific, 12–0112–82 (US) CD11c Hamster-anti-mouse CD11c (clone N418) APC-labeled eBioscience/Thermo Fisher Scientific, 17–0114–82 (US) CD13 Rabbit-anti-CD13 (clone EPR4058) Abcam, ab108310 (UK) CD44 Rat-anti-mouse CD44 (clone IM7) BD Pharmingen, 553131 (US) CD68 Mouse-anti-rat CD68 (clone ED1) Bio-Rad, MCA341R (GER) CD68 Mouse-anti-human CD68 (clone KP1) Dako/Agilent, M081401 (US) Collagen III Goat-anti-type III collagen (antiserum) Southern Biotech, 1330–01 (US) Murine monocytes/macrophages (ER-HR3) Rat-anti-mouse monocyte antigen (clone ER-HR3) BMA Biomedicals, T-2012 (CHE) F4/80 Rat-anti-mouse F4/80 (clone CI:A3–1) Bio-Rad, MCA497G (GER) Fibronectin Rabbit-anti-rat fibronectin (polyclonal) Millipore/Merck AB1954 (GER) KIM-1 Goat-anti-mouse TIM-1/KIM-1/HAVCR (polyclonal) R&D Systems, AF1817 (US) Lipocalin-2 (NGAL) Rat-anti-human lipocalin-2/NGAL (clone 220310) R&D Systems, MAB1757 (US) Lipocalin-2 (NGAL) (for double staining) Rabbit-anti-NGAL (polyclonal) Thermo Fisher Scientific, PA5–79590 (US) Cytokeratin (Pan CK) Mouse-anti-pan Cytokeratin (clone Lu5) Abcam, ab17155 (UK) Proliferation (Ki-67, MIB1) Mouse-anti-human (clone KI-67P) Dianova, DIA-505 (GER) Proliferation (PCNA) Mouse-anti-PCNA (clone PC10) Calbiochem/Merck, NA03 (GER) Sm22 α /Transgelin Rabbit-anti-Transgelin (polyclonal) Abcam, ab14106 (UK) Tamm–Horsfall protein Rabbit-anti-human THP (polyclonal) SantaCruz, sc-20631 (GER) TNFR1 Rabbit-anti-TNFR1 (polyclonal) Abcam, ab19139 (UK) TNFR2 Rabbit-anti-TNFR2 (clone EPR1653) Abcam, ab15116 (UK) Vimentin Mouse-anti-vimentin (clone V9) Dako/Agilent, M0725 (US) Open in a separate window α -SMA, α –skeletal muscle actin; AQ-2, aquaporin-2; CD, cluster of differentiation; GER, Germany; CHE, Switzerland; F4/80, EGF-like module–containing mucin-like hormone receptor–like 1; TIM-1, T-cell immunoglobulin and mucin domain 1; HAVCR, Hepatitis A virus cellular receptor; NGAL, neutrophil gelatinase–associated lipocalin, lipocalin-2; CK, cytokeratin; PCNA, proliferating cell nuclear antigen; SM22 α , Smooth muscle protein 22- α /Transgelin; THP, Tamm–Horsfall protein, uromodulin; TNFR, tumor necrosis factor receptor.

Techniques: Immunohistochemistry, Immunofluorescence, Double Staining